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HEK293T cells were transfected with thepCMV6-ENTRY control (left lane) orpCMV6-ENTRY eGFP(right lane) cDNA for 48hrs and lysed. Equivalent amounts of celllysates (5 ug per lane) were separated bySDS-PAGE and immuno-blotted with 5A2anti-eGFP monoclonal antibody(78bio1006)(1:2000)
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